动物造模,全子宫切除模型 z-bio 2022-09-16 293

　　Objective To establish an experimental animal model of total hysterectomy in BALB/c mice, and to explore and analyze the characteristics and formation mechanism of the model.

　　Methods The patients were divided into blank control group, sham-operated group and model group. The blank control group received no treatment and was fed routinely; the model group underwent abdominal hysterectomy; the sham-operated group only had skin incision to expose the uterus. , the body weight changes of mice in each group were dynamically observed, and the content of estradiol in peripheral blood and the histological changes of ovary were detected at the end of 4 weeks.

　　Results All experimental mice in each group survived after operation, and the general symptoms were good. No obvious inflammation was found in the incision, and the incision was completely healed. There was no significant difference in the preoperative weight of the experimental mice in each group. The body weight of the mice in the model group was higher than that in the control group and the sham-operated group (P<0.05); the peripheral blood estradiol content of the mice in the model group was significantly lower than that in the control group and the sham-operated group (P<0.01); the blank control group and the sham-operated group There were no abnormal histological changes in the ovary of the mice in the operation group, while the model group had abnormal histological changes such as irregular follicle shape, loose granulosa cells and apoptosis.

　　Conclusion This experimental animal model can well simulate the clinical manifestations such as ovarian function decline after clinical operation, and is an ideal animal model for studying complications after hysterectomy and screening drugs.