动物造模,维吾尔医异常黑胆质证大鼠模型 z-bio 2022-09-16 278

　　OBJECTIVE: To evaluate and discuss the biological markers of the rat model of abnormal black gallbladder syndrome in Uyghur medicine by combining the observation of external characteristics with the study of urine metabolome.

　　Methods: The rat animal model was established by the Uyghur medical theory of abnormal black cholestasis, and various external indicators such as hair, tongue, sleep, feces, emotion, weight growth rate, etc. were observed and scored. Urine was tested and analyzed.

　　Results: Compared with the control group, the external characteristics of the rats in the abnormal black gallbladder syndrome model group changed significantly, including dry and hard feces, decreased urine output and darkened color, dry fur, dark purple tongue coating with ecchymosis, and weight growth rate. Decreased, etc.; the contents of 23 metabolites in urine were significantly decreased, including propionate, lactate, alanine, pyruvate, acetic acid, acetamide, glycoprotein, acetone, formin, sarcosine, ornithine, Glycine, creatine, creatinine, creatinine, β-galactose, urocanate, tyrosine, phenylalanine, hippuric acid, amino hippuric acid, formic acid and lysine, etc., while urea, citric acid The contents of four metabolites including allantoin and α-ketoglutarate were significantly increased.

　　CONCLUSION: During the occurrence and development of abnormal black gallbladder syndrome, rats in the model group not only have obvious changes in external phenotype, but also have obvious changes in various internal metabolic pathways. The obvious abnormality of urine metabolites may be related to abnormal black gallbladder syndrome. of biological mechanisms.