动物造模,创伤后应激障碍模型 z-bio 2022-09-16 436

　　OBJECTIVE: To establish a model of post-traumatic stress disorder by single prolonged stress, and to observe the effect of solitary rearing on SPS model rats.

　　METHODS: Thirty-six rats were randomly divided into normal group, SPS model group and SPS combined solitary group, SPS model group and SPS combined solitary group were given SPS for modeling, and SPS combined with solitary group was given separate feeding after modeling. After 7 days of model establishment, the rats' body weight gain, the total movement distance, the times of crossing the grid and the single maximum movement distance in the open field test, and the number and time of freezing behavior in the standing behavior test were measured.

　　Results: Compared with the rats in the normal group, the weight gain and the maximum single exercise distance of the rats in the SPS model group were significantly decreased (P ＜ 0.01), the total exercise distance and the number of crossings were decreased (P ＜ 0.05). The number and time of freezing behavior in the freezing behavior test increased significantly (P < 0.01); the weight gain of the rats in the SPS combined isolation group decreased (P < 0.05), and the number and time of freezing behavior increased in the freezing behavior test. (P < 0.05). Compared with the SPS model group, the total movement distance, the number of crossings and the single maximum movement distance in the open field experiment of the SPS combined solitary group increased (P < 0.05).

　　Conclusion: In this experiment, the rat model of post-traumatic stress disorder was successfully replicated by a single prolonged stress, and the anxiety state of the SPS model was improved by 7-day solitary rearing.