动物造模,高血压合并高脂血症大鼠模型 z-bio 2022-09-16 478

　　OBJECTIVE: To construct a rat model of hypertension complicated with hyperlipidemia by feeding spontaneously hypertensive rats (SHR) with high-fat diet, and to evaluate the damage of the heart and kidney, so as to provide a useful reference for the treatment of hypertension complicated with hyperlipidemia. The evaluation provides reference to pharmacodynamic animal models and related methods.

　　Methods: Three-week-old SHRs were randomly divided into blank control group and model group; Wistar-Kyoto (WKY) rats with normal blood pressure of the same age were selected as model control group. The blank control group and the model control group were fed with normal maintenance diet, and the model group was fed with high-fat diet to induce SHR to produce hypertension and hyperlipidemia. During the modeling period, the blood pressure and body weight of the rats in each group were measured regularly, and the blood pressure, blood lipid and body weight of the rats were measured at the end point of the modeling, and then sacrificed.

　　Results: After SHR high-fat diet was fed for 23 weeks, the blood lipid level was obviously disordered; the heart and kidney organs showed obvious pathological changes: myocardial cell hypertrophy was obvious, the renal tissue interlobular artery wall was significantly remodeled, and the heart and kidney tissue were severely remodeled. fibrosis.

　　Conclusion: SHR can successfully obtain a rat model of hypertension complicated with hyperlipidemia after being fed with high-fat diet for 23 weeks. The performance is similar, and it is expected to be widely used as a drug for the treatment of hypertension and hyperlipidemia. Or a pharmacodynamic evaluation model of therapeutic drugs for improving the cardio-renal system damage in such complications.