动物造模,腰椎腹侧神经根牵拉模型 z-bio 2022-09-16 307

　　Objective: To study the pathogenic mechanism and drug development of amyotrophic lateral sclerosis, establish a rat model of pulling the fourth lumbar ventral nerve root, and use brain-derived neurotrophic factor as a positive drug to verify the effectiveness of the model.

　　Methods: First, 5 SD male rats were operated on. One week later, immunohistochemical staining was performed with anti-choline acetyltransferase antibody to observe the changes in the number of motor neurons in the anterior horn of the spinal cord. 7-week-old SD male rats were randomly divided into four groups, two groups, model control group and two brain-derived neurotrophic factor treatment groups (prophylactic administration group immediately after operation and therapeutic administration group one week after operation). Anti-choline acetyltransferase immunohistochemical staining to observe the changes in the number of motor neurons?

　　Results: The rats recovered well after the operation, and there was no abnormality in the clinical observation. The staining results showed that after the operation, the motor neurons in the anterior horn of the spinal cord were obviously degenerated and died. Compared with the control group, the neurons treated with brain-derived neurotrophic factor were Root pulling animals, whether prophylactic administration or therapeutic administration after 1 week of operation, achieved good therapeutic effects, and the number of choline acetyltransferase staining positive neurons increased significantly (P<0.0001). Results 17.85% vs. 93.06%; 26.6% vs. 87.27%?

　　Conclusion: The rat model of pulling the fourth lumbar ventral nerve root was successfully established, which provides a valuable animal model for the study of amyotrophic lateral sclerosis.