动物实验,胰岛素 z-bo 2020-08-21 537

　　OBJECTIVE: To study the effect of Susie oil high-fat diet rich in n-3 polyunsaturated fatty acids (PUFA) on insulin sensitivity and related gene expression in insulin resistant rats.

　　Method: The insulin resistance model rats were randomly divided into two groups: high-fat group (HF) and perilla oil group (perilla oil group, PO). PO is 20% of the ratio of sesame oil and HF lard. After 4 weeks, the insulin sensitivity of the rats was measured. The content of α-linolenic acid (α-linolenic acid, ALA) in rat serum was detected by gas chromatography. Using western blotting, we detected the protein expression of skeletal muscle glucose transporter 4 (glucose transporter 4, GLUT4) and insulin receptor substrate 1 (insulin receptor substrate 1, IRS-1), and the backbone real-time PCR method Expression of Glut4 and Irs-1 mRNA in muscle.

　　Results: After 4 weeks of administration of Susie oil, compared with the HF group, the ALA intake and serum ALA content of the PO group increased, the expression of GLUT4 protein and mRNA increased significantly, while the expression of IRS-1 protein and mRNA decreased significantly ; The results of hyperinsulinemia-hyperglycemia clamp experiment showed that there was no significant difference in insulin sensitivity between the two groups of rats.

　　Conclusion: ALA (0.556g/d) high-fat diet intervention can regulate the expression of GLUT4 and IRS-1 in skeletal muscle of insulin-resistant rats, but cannot regulate insulin sensitivity.