动物造模,食蟹猴肌肉萎缩模型 z-bio 2022-09-16 335

　　Objective: To establish an animal model of cynomolgus monkey muscle atrophy by restricting lower extremity activities, and to establish a method for evaluating the model?

　　Methods: The right lower limb (ankle joint to knee joint) of cynomolgus monkeys was immobilized with polymer fiber bandages for 15 weeks. During the period, body weight, bilateral calf circumference and lower limb volume were measured every two weeks. Gastrocnemius MRI, muscle biopsy at 15 weeks?

　　Results: After 15 weeks of modeling, the body weight of animals did not change significantly. The circumference of right calf, leg circumference of right thigh, and volume of right lower limb were significantly lower than those before modeling (P<0.05). Serum creatinine and blood sugar? Alkaline phosphatase was significantly decreased ( P < 0.05), and serum cholesterol level was significantly increased ( P < 0.05); MRI showed that the right gastrocnemius muscle atrophy, and the volume of the right gastrocnemius muscle was reduced compared with that before modeling; muscle tissue biopsy showed that the right gastrocnemius muscle was atrophied. Gastrocnemius muscle atrophy, muscle fiber thinning; gastrocnemius muscle fiber cross-sectional area reduced?

　　Conclusion: The muscle atrophy model was made by restricting the activities of the lower limbs of cynomolgus monkeys by external fixation. The modeling cycle was appropriate and the operation was strong. The evaluation methods of leg circumference, lower limb drainage volume, biochemical indicators, MRI imaging and muscle biopsy were used to evaluate the model. Is it possible to simply and objectively evaluate the cynomolgus monkey muscle atrophy model?