动物造模 z-bio 2022-09-24 210

　　Objective: Intrauterine growth retardation is manifested in the way of low birth weight of neonates? Establish IUGR model by three methods commonly used in experiments, and provide reference for the establishment of IUGR model in scientific research by comparing and analyzing experimental data?

　　Methods: Twenty pregnant rats on the 0th day of gestation were randomly divided into four groups, with 5 rats in each group. Three groups were treated with alcohol intervention (A), uterine artery ligation (U), and low-protein diet (LP). The IUGR rat model was established by other methods. The fourth group was the normal control group (CON). The fetuses were harvested by cesarean section on the 20th day of pregnancy, and the body weight, brain weight, placenta weight and kidney weight of the fetuses were weighed. The body length and tail length were measured, and the incidence rate of IUGR, survival rate, brain specific gravity, placental specific gravity, etc. were calculated; each group randomly continued to raise the fetal mice delivered by cesarean section, and measured their body at 3 weeks, 6 weeks, and 12 weeks. Mass? Perirenal fat mass and some organ mass?

　　Results: The average body weight of the three groups of fetal rats in alcohol intervention method, uterine artery ligation method and low-protein diet method was significantly lower than that in the normal control group (P < 0.05), and the incidence of IUGR in the fetal rats was significantly higher than that in the normal control group (P < 0.05). 0.05), the survival rate of fetal rats in the uterine artery ligation group was lower than that in the other two groups. After the rats were fed for 3 weeks, the average body weight of the rats in the experimental group was significantly lower than that in the normal control group (P < 0.05). The average body weight of the rats in the group was still lower than that of the normal control group, and the difference was not significant (P > 0.05). P < 0.05)?

　　Conclusion: All three modeling methods can successfully establish the IUGR model. Among them, the IUGR animal model established by the low-protein diet group has a high incidence rate and a low stillbirth rate, and is suitable for model research; in terms of catch-up growth, the alcohol intervention group and the low-protein diet The average body weight of the diet group was lower than that of the normal control group during the postpartum period and lactation period of the cesarean section rats, but the growth rate after lactation was much higher than that of the normal control group, indicating that the two groups of experimental groups had catch-up growth.