动物造模,氟中毒大鼠模型 z-bio 2022-09-24 246

　　Objective To construct an animal model of male and female fluorosis rats, and to explore the gender differences of fluorosis.

　　Methods Forty-eight clean-grade SD rats weighing about 150 g were selected and divided into control group and fluoride-exposed group. The model group was given free fluoride-containing feed with a fluoride content of 100 mg/kg. The daily conditions of the rats were dynamically observed every three days. Changes in body weight and dental fluorosis, a group of rats were sacrificed by taking blood from the femoral artery when all the female rats and male rats developed dental fluorosis on 70 days and 110 days, respectively. Fluorine content?

　　Results There was no dental fluorosis in the male and female rats in the control group during the whole experiment. The first appearance of dental fluorosis in the female and male rats in the fluoride exposure group was about 60 days and 80 days after exposure to fluoride. About 20 days after exposure to fluoride? Male and female rats entered the rapid growth period about 30 days after exposure to fluoride, and there was a significant difference in body weight between male and female rats (P < 0.01). Compared with the control group? With the prolongation of fluoride exposure time, the urine fluoride and bone fluoride of male and female rats gradually increased, and the fluoride exposure group was significantly higher than the control group, and the difference was significant (P<0.01). Positive correlation? Male mice have higher urinary and bone fluoride than females, and the difference is significant (P < 0?? 01). Urine and bone fluoride are affected by gender?

　　Conclusion The body weight of male and female rat models of fluorosis, the time of dental fluorosis, and the levels of urinary fluoride and bone fluoride are different between genders?