动物造模 z-bio 2022-09-24 281

　　Objective To study the effect of Xishuntongshuan Capsule on the rat model of chronic cerebral ischemia and its influence on the blood-brain barrier.

　　Methods SD rats were used to create a chronic cerebral ischemia model with bilateral common carotid artery ligation. The water maze was used to evaluate the learning and cognitive function of the rats, Nissl staining was used to observe the neurons in the hippocampus, and immunofluorescence staining was used to observe the damage of the blood-brain barrier.

　　Results Xishutongshuan Capsules can improve the decline of learning and cognitive function in rats after chronic cerebral ischemia, reduce the decrease of Nissl bodies and IgG leakage in hippocampal neurons, and reduce the levels of MMP-9, claudin-5 and occludin in plasma. , to increase the expression of claudin-5 and occludin in brain tissue.

　　Conclusion Xishuntongshuan Capsules can reduce blood-brain barrier damage and improve learning and cognitive function after chronic cerebral ischemia.