动物造模 z-bio 2022-09-24 297

　　Objective To establish a depression model in SD rats through chronic mild and unpredictable stimulation, and to preliminarily explore the effect and mechanism of different styles of music on improving depression-like behavior in SD rats.

　　Methods CUMS rats were constructed by chronic unpredictable stimuli lasting 4 weeks after solitary care. Thirty-nine CUMS depressed rats were screened by sugar water preference test and forced swimming test. They were randomly divided into model group, light music group and classical music group. A control group without any stimulation was set up as blank control. The light music group and the classical music group each played the music of Hisaishi and Mozart for 4 hours every day, and then performed the SPT, FST and O-maze test (0-maze) immediately after 3 weeks. Materials were collected immediately after the test. The structural changes of the hippocampus were observed by HE staining, the expression levels of brain-derived neurotrophic factor were detected by immunohistochemistry and Western Blot, and the contents of corticosterone and pentahydroxyl in the hippocampus were detected by liquid chromatography-tandem mass spectrometry. The changes of BDNF, CORT, 5-HT and IL-1β in peripheral blood were detected by ELISA.

　　Results Compared with the model group, the percentage of sugar water preference of the rats in the light music group and the classical music group were increased (P<0.05), the FST immobility time was shortened (P<0.01), the number of times and the dwell time of entering the open arm of the O maze were both increased (P<0.05). increased (P<0.05). Compared with the model group, the hippocampal structure of the light music group and the classical music group was complete and neatly arranged, and the expression level of BDNF was increased (P<0.01). After music intervention, serum BDNF levels were significantly increased in both groups (P<0.01). The CORT content in the hippocampus of the two music groups was decreased (P<0.01), and the serum CORT content in the classical music group was significantly lower in the classical music group (P<0.01). Serum 5-HT level was increased in the light music group (P<0.01), but there was no significant difference in the classical music group. Serum IL-1β concentrations in both music groups were significantly decreased (P<0.01).

　　Conclusion Both classical music and light music can improve the behavior of depression model rats to a certain extent.