动物造模 z-bio 2022-09-24 271

　　Objective To study the effect of compound probiotics on intestinal mucosal immune function in mice.

　　Methods A total of 80 28-day-old BALB/C mice were selected and given different doses (2.5, 5, and 10 g/kg body weight) of compound probiotics by gavage. Blood samples and jejunum tissue were taken, and immune organs were taken for determination of immune organ index. The content of lysozyme in blood was detected by turbidimetric method, the contents of immune cytokines (IL-4, IL-10, IFN-γ) and SIgA in mouse jejunum mucosa were detected by ELISA method; the immune cells of mouse jejunum mucosa were detected by fluorescence quantitative PCR. factor mRNA expression levels.

　　Results (1) Different doses of the compound probiotics could increase the content of immune cytokines and SIgA in the intestinal mucosa of mice and the expression level of immune cytokine mRNA. On the 14th day, the contents and mRNA expression levels of immune cytokines IL-4, IL-10, IFN-γ and SIgA in the jejunal mucosa of mice in the middle-dose group were significantly higher than those in the control group (P < 0.01). On the 28th day, the content of IL-4 in the jejunal mucosa of the low-dose group and the middle-dose group was significantly increased (P < 0.01); the content and mRNA expression of IL-10, IFN-γ and SIgA in the middle-dose group were significantly higher than those in the control group. The mRNA expression levels of IL-4, IL-10 and IFN-γ in the middle and high dose groups were higher than those in the control group to varying degrees (P < 0.05 or P < 0.01); The mRNA expression levels of IL-4 and IL-10 in the low-dose group were not significantly different from those in the control group (P > 0.05). (2) On the 14th day, the immune organ index of the middle dose group was significantly higher than that of the control group (P < 0. 05), and on the 28th day, the immune organ index of each experimental group was significantly higher than that of the control group (P < 0. 05). (3) The content of lysozyme in each experimental group was significantly higher than that in the control group (P<0.05).

　　Conclusion The compound probiotics can promote the growth of mice, increase the immune organ index of mice, improve the content of lysozyme in the blood of mice and the immune function of intestinal mucosa, and reduce the occurrence of intestinal infectious diseases.