动物造模 z-bio 2022-09-24 276

　　Objective To investigate the regulatory effect of curcumin and/or aerobic exercise on intestinal function in rats with dyslipidemia.

　　Methods Forty 5-week-old male SPF SD rats were randomly divided into normal diet + quiet group (CON group), high-fat diet + quiet group (HDC group), high-fat diet + curcumin + quiet group (HDM group) , high-fat diet + aerobic exercise group (HDE group) and high-fat diet + curcumin + aerobic exercise group (HDME group). The high-fat diet groups were fed with high-fat feed, and the normal diet group was fed with maintenance feed. From the third week, the HDM and HDME groups were given 200 g/(kg·d) curcumin by gavage, and the other groups were given an equal volume of 0.5% sodium carboxymethyl cellulose by gavage; HDE and HDME groups were given exercise intensity of 70 % ~ 75% of the maximum oxygen uptake aerobic exercise, the other groups did not have any exercise intervention. 24 h after the 6-week intervention, blood was collected from the abdominal aorta, and cecal tissue and cecal contents were collected. The blood lipid level was detected by automatic biochemical analyzer; the morphology of rat cecum was observed after HE staining; the protein expression level of zona occludens-1 (ZO-1) in cecal tissue was detected by Western Blot; 16S rDNA gene sequencing technology Detection of intestinal flora distribution in cecal contents.

　　Results Curcumin and/or aerobic exercise could reduce serum total cholesterol and triglyceride in rats with dyslipidemia (P < 0. 05); up-regulated the expression level of ZO-1 protein in cecal tissue (P < 0. 05) and improved tissue Morphology; improved the diversity and uniformity of intestinal flora (P < 0.05).

　　Conclusions Curcumin and/or aerobic exercise can effectively regulate intestinal flora and improve the integrity of intestinal mucosal barrier and intestinal function in rats with dyslipidemia. Among them, the combined intervention has the best effect.