动物造模 z-bio 2022-09-24 313

　　Objective To investigate the neuroprotective effect of Dendrobium officinale extract in a mouse model of Parkinson's disease induced by 1-methylv-4-phenyl-1,2,3,6-tetrahydropyridine.

　　Methods Mice were randomly divided into control group, model group, Madopar group (positive drug group), DNL (100, 200, 300 mg/kg) group. d), 7 d) A subacute mouse PD model was established, and gait analysis experiments, pole climbing experiments, and suspension experiments were performed to evaluate the effect of DNL on the abnormal movement of PD mice; enzyme-linked immunosorbent assay (ELISA) was used to detect each group. Changes in the expression of tumor necrosis factor-α, interleukin-6 and interleukin-1β in the striatum and cortex of mice; Western blotting was used to detect caspase-3 and caspase in the striatum of mice in each group Expression of -9.

　　Results Compared with the control group, the propulsion index and the gait index of support phase in the model group had significant changes (P<0.05). In the left rear, right front and right rear propulsion index, the left rear, right front and right rear support phases of the group were significantly improved (P<0.05); compared with the control group, the model group increased in the upper half of the pole and the total time (P<0.01, P<0.001), there="" was="" no="" significant="" change="" in="" u-turn="" time="" and="" the="" second="" half="" p="">0.05); compared with the model group, the DNL (300 mg/kg) group could improve the first half time (P<0.05). 200="" madopar="" and="" dnl="" p="">0.05); compared with the control group, the suspension score of the model group was decreased (P<0.05), compared with the model group, DNL (300 mg/kg) Can increase the suspension score (P<0.05), 200="" madopar="" and="" dnl="" p="">0.05); compared with the control group, TNF-α, IL-1B.ⅡL in the striatum of mice in the model group The content of -6 increased (P<0.05), and the protein expression levels of caspase-3 and caspase-9 increased significantly (P<0.001); The expression levels of caspase-3 and caspase-9 decreased (P<0.05).

　　Conclusion DNL can improve MPTP-induced behavioral changes in PD mice, reduce the inflammatory response in brain tissue, and inhibit nerve cell apoptosis. It has good potential in the development of PD drugs and functional foods.