动物造模 z-bio 2022-09-27 268

　　Objective To study the effect of betel nut on depressive-like behavior in mice and its mechanism.

　　Methods The behavioral despair model and reserpine antagonism model were established respectively. Male ICR mice were divided into betel nut low, medium and high dose groups (160, 320, 640 mg/kg) and positive drug group (fluoxetine, 20 mg/kg). )), continuous intragastric administration for 14 d. Behavioral tests were used to evaluate the antidepressant activity of betel nut by open field test (OFT), tail suspension test (TST) and forced swimming test (FST) in mice; Effects of monoaminergic nervous system function and altered levels of oxidative stress. The LC-MS/MS analysis method established by our research group was used to determine the content of neurotransmitters in mice; the activities of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were used to reflect betel nut resistance. oxidative stress levels.

　　Results There was no significant difference in body weight between the groups. The results of the open field experiment showed that betel nut had no effect on the spontaneous activity of mice. The middle and high dose groups of betel nut can significantly reduce the immobility time of tail suspension (P<0.05, P<0.01), and the low and high dose groups can significantly reduce the immobility time of mice in the forced swimming test (P<0.05). In the reserpine antagonism experiment, compared with the model group, the betel nut low and high dose groups could antagonize the decrease in body temperature of mice caused by reserpine (P<0.01), and the three dose groups could significantly antagonize the reserpine-induced reduction of body temperature in mice (P<0.01). Mice had ptosis (P<0.05). Compared with the reserpine antagonism model group, the content of 5-HT in the brain tissue of mice in the high-dose betel nut group (P<0.01), the DA content in the low- and high-dose groups of betel nut (P<0.05, P<0.01); The level of GABA in the middle-dose group (P<0.05) was significantly increased; the level of 5-HIAA in the middle-dose and high-dose groups of betel nut was significantly decreased (P<0.01). The activities of SOD and CAT were significantly enhanced in the three dose groups of areca nut (P<0.01, P<0.01); the content of MDA in the middle and high dose groups of betel nut was significantly decreased (P<0.01).

　　Conclusions Betel nut has good antidepressant efficacy in both stress-type despair model and reserpine antagonist drug model, and does not affect motor activity and body weight. Its antidepressant mechanism may be related to the enhancement of the level of monoaminergic system in the brain and the level of anti-oxidative stress.