动物造模,脑缺血再灌注损伤模型 z-bio 2022-09-27 348

　　Objective To explore the intervention effect and mechanism of down-regulation of AQP-4 on cerebral ischemia-reperfusion injury model rats.

　　Methods 40 SD healthy male rats were selected, 10 were divided into normal group, and the remaining 30 SD healthy male rats were divided into model group, down-regulated AQP-4 group, and up-regulated AQP-4 group. The four groups of rats were intervened respectively. . Western blot was used to detect the expressions of AQP-4, BMP4 and P-Smad, and nephelometry was used to detect the levels of TNF-α and IL-1β. The learning and memory ability and neurological function of the rats in each group were compared. Brain tissue water content was detected.

　　Results Compared with the normal group, the escape latency at each time point of the other three groups of rats was longer, and the number of crossing the platform was lower (P<0.05). Compared with the normal group, the model group, the up-regulated AQP-4 group and the down-regulated AQP-4 group had higher neurological function scores, higher brain tissue water content Compared with the model group and the up-regulated AQP-4 group, the neurological function score of the down-regulated AQP-4 group decreased, and the water content of the brain tissue decreased (P<0. 05); compared with the normal group, other The levels of TNF-α and IL-1β in the three groups were increased (P<0.05); compared with the model group and the up-regulated AQP-4 group, the levels of TNF-α and IL-1β in the down-regulated AQP-4 group decreased (P<0.05); Compared with the normal group, the expression of AQP-4 in the other three groups increased, and the expression of BMP4 and P-Smad decreased (P<0.05); compared with the model group and the up-regulated AQP-4 group , the expression of AQP-4 decreased, and the expressions of BMP4 and P-Smad increased in the down-regulated AQP-4 group (P<0.05). Compared with the normal group, the contents of Na+ and P-dp in the other three groups increased (P<0.05). 05); compared with the model group and the up-regulated AQP-4 group, the Na+ and P-dp contents of the rats in the down-regulated AQP-4 group decreased (P<0.05).

　　Conclusion Down-regulation of AQP-4 can intervene in cerebral ischemia-reperfusion injury model rats through the BMP4/Smad pathway, repair the blood-brain barrier in the brain, improve the learning and memory ability of the rats, and reduce the inflammatory response of the rats.