动物造模 z-bio 2022-09-27 343

　　Objective To establish a method for intestinal colonization of C57BL/6 mice by Campylobacter jejuni through antibiotic-induced gut microbiome depletion.

　　Methods Thirty-six C57BL/6 mice were divided into normal group, control group and experimental group. After continuous intragastric administration of cefoperazone sodium and sulbactam sodium (50 mg/mL) for 2 d, 200 μL of Campylobacter jejuni were intragastrically administered. On the 1st to 3rd days, the intestinal contents were collected for 16S rDNA analysis of bacterial diversity, and the animal feces from 1 to 7 days after modeling were collected, and the fecal Campylobacter jejuni HipO gene was detected by probe method qPCR. On ~3 days and the seventh day, the intestinal tissues of mice were detected by immunofluorescence method to detect the colonization of Campylobacter enterica, and HE staining was used to observe the pathological changes.

　　Results After modeling, Lactobacillus in the ileum, Bacteroides in the cecum and colon of the experimental group were suppressed, the relative abundance of Enterococcus was higher, and the higher abundance of Campylobacter was detected in the experimental group on the first day. On the 1st to 3rd day after modeling, a higher copy number of Campylobacter jejuni could be detected in the feces of animals in the experimental group, and obvious immunofluorescence-labeled Campylobacter jejuni could be seen in the intestinal lumen, while the intestinal mucosa was basically intact. No obvious inflammatory cell infiltration was seen.

　　Conclusion The microbiome depletion induced by cefoperazone sodium and sulbactam sodium can promote the short-term colonization of C. jejuni in the intestinal tract of mice.