动物造模,药物性肝损伤 z-bio 2022-09-28 308

　　Objective Paijiangcao is the whole rooted herb of the perennial herbs of the family Paiaceae, Huanghuabaijiang and Baihuabaijiang, with various pharmacological activities. The purpose of this study was to investigate the protective effect of Patrinia on acetaminophen-induced drug-induced liver injury in rats.

　　Methods The rats were randomly divided into 6 groups by tail vein injection of acetaminophen: control group, model group, low-dose Patrinia spp. . Simultaneously with the modeling, the low, medium and high dose groups of Patrinia spp. were given 4, 8, and 16 g/kg of Patrinia spp. by gavage every day. The model group was given an equal volume of normal saline by gavage for 30 consecutive days. Liver index was calculated; HE staining was used to detect the degree of liver tissue pathological damage; kits were used to detect the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in serum; enzyme-linked The levels of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and interleukin 1β (IL-1β) were detected by immunosorbent assay; total superoxide dismutase (SOD), total superoxide dismutase (SOD), total superoxide dismutase (SOD), Malondialdehyde (MDA), glutathione peroxidase (GSH-Px) content.

　　Results Compared with the control group, the liver index of the model group was significantly increased (P<0.05), the levels of AST, ALT, and ALP were significantly increased (P<0.05), and the levels of TNF-α, IL-6, and IL-1β were significantly increased. (P<0.05), the contents of SOD and GSH-Px were significantly decreased (P<0.05), and the contents of MDA were significantly increased (P<0.05). Compared with the model group, the liver index of the medium and high dose groups and the bifendate group were significantly decreased ( P<0.05), the degree of pathological damage was significantly improved, and the levels of AST, ALT and ALP were significantly decreased (P<0.05). The levels of TNF-α, IL-6 and IL-1β were significantly decreased (P<0.05), the contents of SOD and GSH-Px were significantly increased (P<0.05), and the content of MDA was significantly decreased (P<0.05).

　　Conclusions Paijiangcao has a protective effect on acetaminophen-induced drug-induced liver injury in rats by improving the pathological damage of liver tissue, inhibiting the release of inflammatory factors, and improving oxidative stress.