动物造模 z-bio 2022-09-28 373

　　Objective To investigate the effect of sevoflurane labor analgesia on hypoxia in neonatal rats based on the mitogen-activated protein kinase (MAPK) signaling pathway.

　　Methods The model group and sevoflurane group were used to establish labor asphyxia model. The model group was given 50% oxygen by volume, and the sevoflurane group was given 50% oxygen and 2.5% sevoflurane. The spontaneous delivery group was given spontaneous delivery. . To evaluate the learning and memory ability of newborn mice, detect serum superoxide dismutase (SOD), malondialdehyde (MDA) levels, the number of Nissl bodies in hippocampal CA1 area of brain tissue, MAPK in brain tissue, B-cell lymphoma-2 gene ( Bcl-2), Bcl-2-related X protein (Bax), cysteine protease-3 (Caspase-3) mRNA and protein expression.

　　Results Compared with the model group, the neonatal mice in the sevoflurane group had prolonged residence time in the target quadrant, increased platform crossing times, increased serum SOD, decreased MDA, increased number of Nissl bodies in hippocampal CA1 region, and increased Bcl-2 mRNA and protein levels in brain tissue. The expression levels and Bcl-2/Bax increased, while the mRNA and protein expressions of MAPK, Bax, Caspase-3 decreased, and the differences were statistically significant (P<0.05).

　　Conclusion Sevoflurane labor analgesia can effectively improve the hypoxic-ischemic brain injury in neonatal rats, which may play a role in brain protection by inhibiting the MAPK signaling pathway, thereby inhibiting the damage of hippocampal neurons.