动物造模 z-bio 2022-09-28 331

　　Objective To intervene ovalbumin (OVA)-sensitized asthmatic mice with Qingre Runzao Oral Liquid, and to observe its effect on the number of type II innate lymphocytes (ILC2s) and its upstream and downstream cytokines in asthmatic mice.

　　Methods BALB/c mice (female) were divided into blank control group, model control group, positive control group (dexamethasone group, 1 mg/(kg·d)), Qingre Runzao oral liquid low and high (4.32, 8.64 g/(kg·d)) dose group, a total of 40 rats. OVA was used as a sensitizer to induce asthma mouse model, and HE staining was used to observe the pathological changes of mouse lung tissue. -25, IL-33, TSLP, IL-4, IL-5 and IL-6 levels; immunohistochemical method to detect the expression of cytokines IL-33 and IL-5 upstream and downstream of ILC2s in lung tissue; flow cytometry and immunoassay Double fluorescent staining was used to detect the number and generation of ILC2s in lung tissue.

　　Results Compared with the blank control group, the model control group showed pathological changes of inflammatory cell infiltration and bronchial wall thickening in the lung tissue, and the upstream and downstream cytokines Eotaxin, IL-25, IL-33, TSLP, IL-4 of ILC2s in BALF , IL-5 and IL-6 levels increased (P<0.01), the expressions of upstream and downstream cytokines IL-33 and IL-5 of ILC2s in lung tissue increased (P<0.01), and the number and production of ILC2s increased (P<0.01). P<0.01). Compared with the model control group, Qingre Runzao Oral Liquid at low and high doses significantly improved the pathological damage of lung tissue in asthmatic mice, decreased the expression of upstream and downstream cytokines of ILC2s in BALF (P<0.01), and down-regulated the upstream and downstream cytokine IL-2 of ILC2s in BALF. 33 and IL-5 expression (P<0.05, P<0.01) and decreased the number and production of ILC2s (P<0.01).

　　Conclusion Qingre Runzao Oral Liquid can reduce the number of ILC2s and its upstream and downstream cytokines in OVA-induced asthma mice, and down-regulate the expressions of IL-33 and IL-5 in lung tissue, thereby preventing and treating asthma.