动物造模,非酒精性脂肪性肝病 z-bio 2022-09-28 276

　　Objective To investigate the effect of Spirillum hepaticum infection on nonalcoholic fatty liver disease induced by high-fat diet.

　　Methods Twenty six week old male BALB/c mice were randomly divided into four groups: blank control group, HFD group and H Group h and H H+HFD group. H. H+HFD group infected with H H later, they were fed with high-fat diet. The mice in each group were killed after 12 weeks of feeding. The contents of triglyceride (TG), alanine transaminase (ALT) and aspartate aminotransferase (AST) in serum of the mice in each group were detected. The liver lesions were evaluated by oil red O staining, sirius red staining, hematoxylin eosin staining, real-time fluorescent quantitative PCR and immunohistochemistry.

　　Results The levels of TG, ALT and AST in serum of mice in the H.h+HFD group were significantly higher than those in the blank control group (all P<0.05), and higher than those in the H, h and HFD groups (all P<0.05). H. In the liver of h+HFD mice, fat deposition, ballooning degeneration and dot necrosis of hepatocytes, and large amount of collagen fibers were observed; H. In group h, inflammatory cells were aggregated and a few collagen fibers were deposited; In HFD group, lipid droplets accumulation, lipoid degeneration and a small amount of collagen fiber deposition were observed. H. Interleukin-6 (IL-6) and IL-1 in liver of mice in h+HFD group β、 Transforming growth factor- β （transforming growth factor- β， TGF- β） And tumor necrosis factor- α （tumor necrosis factor- α， TNF- α） The mRNA transcription level was significantly higher than that of the other three groups (all P ≤ 0.05), and α- SMA and Collagen I protein were also expressed.

　　Conclusion H, h infection can promote the development of nonalcoholic fatty liver disease induced by high-fat diet in mice.