动物造模 z-bio 2022-09-28 321

　　Objective To study the effect of Oct4 and Cdx2 knockdown on the gene expression related to the differentiation of bovine ICM and TE, and to explain the role of Oct4 and Cdx2 in the early development of bovine embryos.

　　Methods Knock down Oct4 or Cdx2 gene of bovine embryos by RNA interference. The effects of knockdown of Oct4 or Cdx2 gene on the early development of bovine embryos and the expression of related genes were detected by real-time fluorescent quantitative PCR and immunofluorescence staining.

　　Results The specific siRNA targeting Oct4 or Cdx2 gene could inhibit the expression of Oct4 or Cdx2 in bovine embryos. Oct4 knockdown had no effect on embryo cleavage rate and 8-cell rate, but significantly reduced embryo blastocyst rate and mRNA level (P<0.05). Cdx2 knockdown inhibited Oct4 mRNA expression (P<0.05).

　　Conclusion Oct4 controls the differentiation of bovine embryos by regulating the expression of Cdx2, Sox2 and Nanog.