动物造模 z-bio 2023-04-25 507

　　Objective To explore the differences of physiological and biochemical indexes of different oils in mice under different feed models, and to provide experimental basis for the selection and evaluation of feed oils in animal models and the use of oils for human health.

　　Methods One hundred and twenty C57BL/6J male mice were used as the research object. According to the energy supply of fat, two levels were set: 20% (low energy supply level of fat) and 30% (high energy supply level of fat, the recommended proportion of energy supply of daily dietary fat should not exceed 30%). At each energy supply level of fat, lard oil (LA), rapeseed oil (RA), olive oil (OL) and mixed oil of lard and rapeseed oil (mass ratio 1 ∶ 1) (BO) were selected respectively. After 16 weeks of feeding, the effects on growth performance, fat deposition, serum biochemical indexes and blood glucose of mice were analyzed.

　　Results Under the condition of low lipid energy supply, the final body weight of mice in OL group decreased significantly, the accumulation of perirenal fat and epididymal fat decreased significantly (P<0.01), and the Lee's index was the lowest. Under the high level of oil energy supply, the body weight, perirenal fat and epididymal fat accumulation of mice in OL group and RA group decreased significantly (P<0.01). In OL group, the liver index (P<0. 01) was significantly increased under the two levels of oil supply, while in RA group, the kidney index (P<0. 05) was significantly increased under the low level of oil supply, while in LA and RA groups, the kidney index (P<0. 01) was significantly increased under the high level of oil supply. Under the high lipid energy supply level, the serum TC content of RA group was significantly lower than that of other groups (P<0.05), and the TG content of OL group was the lowest. From the HDL-C/LDL-C ratio, the HDL-C/LDL-C ratio of RA group was significantly higher than that of the other three groups (P<0.05) under="" the="" low="" lipid="" energy="" supply="" and="" there="" was="" no="" difference="" between="" ra="" group="" ol="" high="" level="" p="">0.05). From the GTT and ITT experiments, BO and OL had better regulation of blood glucose homeostasis under low lipid energy supply level, while there was no difference among the four groups under high lipid energy supply level (P>0.05). Although the blood glucose at the end of BO group increased slightly, the overall blood glucose value was low, which did not cause blood glucose regulation disorder.

　　Conclusion The mixed oil of lard and rapeseed oil (1 ∶ 1) can increase the body weight and fat deposition of mice under the high oil energy supply level. Under the two oil energy supply levels, olive oil can reduce the body weight and fat deposition, but it may have adverse effects on liver function. Rapeseed oil has the best effect on the improvement of blood lipid, but it increases the kidney index. None of the four oils has impaired the ability to regulate blood glucose in mice.