动物造模,帕金森病 z-bio 2022-10-18 349

　　Objective To investigate the effects of Fufang Dihuang Granules on the regulation of serum cyclic nucleotides (cAMP, cGMP) and the repair of dopamine neurons in Parkinson's disease mice with yin deficiency and movement wind syndrome.

　　Methods Twenty four male C57BL/6 mice were randomly divided into normal group (Nomal), model group (MPTP+NS) and treatment group (MPTP+CDG) with 8 mice in each group. Normal saline was injected into mice in the normal group, and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was injected intraperitoneally into mice in the model group to establish PD animal models. Both groups were injected once a day for 5 consecutive days. After modeling, the treatment group was given Compound Dihuang Granule (CDG) by gavage once a day for 7 consecutive days. This study verified the TCM syndrome attribute of the model from the aspects of etiology, symptoms, objective index detection, formula test, etc. of PD mice, and discussed the effect of CDG on the expression of cyclic nucleotides and tyrosine hydroxylase in the serum of PD mice with yin deficiency and movement wind syndrome.

　　Results There were significant differences between the model group and the normal group in behavior, ratio of tongue color value, content of serum cyclic nucleotides and content of tyrosine hydroxylase (P<0.05). CDG treatment could significantly improve the behavior and related objective indicators of mice (P<0.05).

　　Conclusion The mouse model of Parkinson's disease with yin deficiency and dynamic wind syndrome prepared by intraperitoneal injection of MPTP is stable and reliable. CDG can regulate the content of cyclic nucleotides in serum and effectively repair the injured dopaminergic neurons.