动物造模,阿尔茨海默病 z-bio 2022-10-19 257

　　Objective To explore and establish a reliable animal model of Alzheimer's disease combined with phlegm and blood stasis syndrome, and to provide the corresponding animal model for the prevention and treatment of AD by traditional Chinese medicine and provide reference for the establishment of animal model of disease combined with syndrome.

　　Methods APP/PS1 double transgenic mice were used as AD model animals, and the pathological state of "blood stasis" was simulated by ice water bath; High fat diet was given to simulate the pathological state of "phlegm"; They are combined to simulate the pathological state of "phlegm and blood stasis". Different groups of mice were given different treatments to establish AD disease model group, AD phlegm syndrome group, AD blood stasis syndrome group and AD phlegm blood stasis interaction group respectively. The same strain non transgenic C57BL/6J mice were used as the control group. After 14 days of modeling, the AD like behavior changes, objective changes of tongue picture, changes of blood rheology and blood lipid, and the differences of related protein content in hippocampus of different groups of mice were detected.

　　Results After 14 days of modeling, compared with the control group, there were significant changes in the behavior of mice in AD groups, and the content of related proteins increased. Compared with the AD model group, the tongue color of the mice in the AD pathological status groups was dark red, the blood rheology indicators in the AD blood stasis syndrome group and the blood lipid indicators in the AD phlegm syndrome group were all increased, and the relevant indicators in the AD phlegm blood stasis interaction group were significantly increased.

　　Conclusion The APP/PS1 double transgenic mice treated with ice water bath and high-fat diet for 14 days can successfully establish a disease syndrome combined animal model of AD phlegm blood stasis syndrome. This method has a high molding rate and is consistent with clinical symptoms, which can provide a stable animal experimental carrier for subsequent related research.