动物造模,帕金森病 z-bio 2022-10-19 361

　　Objective To observe the effects of different doses of protease inhibitor on behavior and tyrosine hydroxylase α- To explore the best condition of chronic model of Parkinson's disease induced by PSI.

　　Methods Thirty male SPF rats were randomly divided into three groups, 10 rats in each group. The dosage of PSI was 3 mg/kg for model group A and 6 mg/kg for model group B, respectively. The control group was injected with dimethyl sulfoxide (DMSO) solution in the same way, the dosage was 3 mg/kg. After 2 weeks of continuous injection every other day (Monday, Wednesday and Friday), observe the behavioral changes of PD rats. At the same time, observe the changes of cells in the substantia nigra area of the midbrain under the light microscope with routine HE staining. Western blot and immunohistochemical staining were used to detect the dopamine neuron tyrosine hydroxylase (TH) in the substantia nigra and substantia nigra area of the midbrain in each group of rats α- The expression of syn changed.

　　Results Compared with the control group, the exercise ability of the two groups in the PSI model group decreased significantly. The behavioral score of the pole climbing test in the model group A was lower than that in the model group B, and the behavioral score of the suspension test was higher than that in the model group B (P<0.05); HE staining showed that the substantia nigra cells in the model group were degenerated; Western blot and immunohistochemical results showed that the expression of TH in substantia nigra of rats was significantly reduced α- The expression of syn increased significantly.

　　Conclusion The PSI rat model can replicate the behavioral and central and peripheral neurodegenerative features of PD, and is an effective model for studying the pathogenesis of PD.