动物造模,肺癌原代细胞脑转移模型 z-bio 2022-10-20 398

　　Objective This study intends to isolate and culture primary small cell lung cancer cells that retain the important features and functions of the original tumor, and build a mouse model of SCLC primary cell brain metastasis on this basis, so as to provide a more clinical animal model for drug development and evaluation of patients with SCLC brain metastasis.

　　Methods Fresh SCLC tumor tissue samples from clinical patients were collected for subcutaneous transplantation in mice. The construction of human tumor xenotransplantation (PDX) model of small cell lung cancer was determined by the tumor bearing condition, tumor tissue size and tumor growth curve of mice. The primary cells of tumor tissue after tumorigenesis were isolated and cultured, and the proliferation and invasion ability of cells were tested by cell scratch test and proliferation test. The brain metastasis model of SCLC was established by injecting primary cells into carotid artery, and the success of the model was determined by clinical manifestations and pathological observation.

　　Results (1) The subcutaneous PDX model of SCLC was successfully constructed. The passage time of the tumor was about 56 days, and the tumor growth curve showed a logarithmic growth trend. (2) The primary L0 cells of SCLC with tumorigenicity were successfully isolated and screened, and it was confirmed that the L0 cells had strong ability of cell migration and proliferation. (3) The brain metastasis model of SCLC was successfully established by carotid injection, and B0 cells with SCLC brain metastasis characteristics were isolated.

　　Conclusion In this study, primary SCLC cells were isolated and cultured, and the brain metastasis model of primary SCLC cells was successfully constructed.