动物实验 z-bo 2020-08-21 592

　　OBJECTIVE: To study the effect of moloniside on the expression of tissue metalloproteinase (TIMP) in rat cortex after ischemia-reperfusion for 7 days.

　　Method: 25 male SD rats were made using the modified Zealonga suture method, and the middle cerebral artery occlusion (MCAO) model, model group, low-dose moloniside group, medium-dose group, and large group after surgery were randomly prepared. Dose group, 5 per group. Three hours later, molonicid was administered in the stomach at doses of 30, 90, and 270 mg/kg once a day. Western blotting and immunofluorescence staining were used to analyze the effect of moloniside on the expression of TIMP in rat cortex 7 days after cerebral ischemia and reperfusion.

　　Results: 7 days after cerebral ischemia and reperfusion, the expression of TIMP protein in the model group was significantly increased compared with the sham operation group (P \u003cu003c0.01); compared with the sham operation group, the difference was statistically significant (P\u003c0. 01). The expression of TIMP protein in Moroniside middle-dose group (90 mg/kg) and high-dose group (270 mg/kg) increased significantly (P\u003c0.05; P\u003c0.01).

　　Conclusion: The rodenticide up-regulates the expression of TIMP in the cerebral cortex, and helps to protect and repair the blood-brain barrier after stroke.