动物造模,胰腺癌原位模型 z-bio 2022-10-20 287

　　Objective To explore the advantages and disadvantages of establishing an in situ model of pancreatic cancer in mice by tumor cell injection and tumor tissue transplantation, and to provide a technical reference for establishing an in situ model of pancreatic cancer in mice for the purpose of precise ablation therapy.

　　Methods Twenty healthy male C57BL/6 mice aged 6-8 weeks were divided into four groups with five mice in each group: Group A was treated with direct injection of Panc02 cell suspension of mouse pancreatic cancer; Group B was injected with mouse pancreatic cancer Panc02 cell suspension containing matrix glue; In group C, pancreatic cancer tissue block transplantation was used; In group D, the mice tumor tissue block hydrogel bonding method was used. After operation, the tumor formation rate, tumor size, degree of abdominal organ adhesion and tumor metastasis of pancreatic cancer in mice of each group were observed. HE staining and immunohistochemical methods were used to detect the tumor histomorphology, Ki67 and Ki67, respectively α Smooth muscle actin（ α- smooth muscle actin， α- SMA).

　　Results The tumorigenic rates of pancreatic cancer in group A, B, C and D were 60%, 80%, 100% and 100% respectively. After 16 days of modeling, the tumor volume of group A mice was (47.80 ± 42.99) mm3, with poor homogeneity; B. The tumor volumes of mice in group C and D were (68.43 ± 16.77) mm3, (105.86 ± 17.25) mm3 and (128.98 ± 13.41) mm3, respectively, which were significantly larger than those in group A (P<0.01) and="" had="" better="" homogeneity.="" the="" rate="" of="" severe="" abdominal="" adhesions="" was="" in="" group="" a="" b.="" there="" no="" c="" d.="" tumor="" metastasis="" found="" significant="" difference="" histology="" among="" four="" groups="" ki67="" -="" relative="" expression="" level="" sma="" protein="" p="">0.05).

　　Conclusion The in situ model of pancreatic cancer in mice established by tumor tissue block transplantation and hydrogel bonding has high tumorigenesis rate, less metastasis, light intraperitoneal adhesion, and does not change the biological characteristics of tumor cells. It is easy to operate and popularize, and has better modeling effect.