动物造模,心肌损伤模型 z-bio 2022-10-20 383

　　Objective To establish a model of myocardial injury induced by isoproterenol in rhesus monkeys.

　　Methods 11 rhesus monkeys were divided into high dose group, low dose group and control group. In the model group, the dose of ISO was 4.6mg/kg and 3.2mg/kg respectively, twice a day; The control group was injected with 1mL of normal saline once a day. Color Doppler ultrasound imaging was performed on the 1st, 3rd, 5th, 7th day and the 2nd, 3rd, 4th, 5th week after injection respectively: left ventricular septal thickness (IVSTd), left ventricular diameter (LVEDD), left ventricular posterior wall thickness (LVPWTd) at the end of diastolic phase, left ventricular septal thickness (IVSTs) at the end of systolic phase, left ventricular diameter (LVESD), left ventricular posterior wall thickness (LVPWTs), and left ventricular short axis shortening rate (FS), ejection fraction (EF), left ventricular mass (LVM); ECG changes detected by electrocardiograph; At the same time, the blood physiological and biochemical indexes such as ALT, AST, CK, CK-MB, LDH, PLR and NLR were detected.

　　Results In the high-dose group, AST, ALT, LDH, CK, CK-MB, PLR, NLR increased significantly within one week after administration; IVSTd, IVSTs, LVPWTd, LVPWTs, LVEDD, LVESD increase, EF, FS decrease. Compared with the high dose group, the low dose group has no significant change. The results of ECG showed that T wave and ST segment changed continuously from the fifth week after administration.

　　Conclusion Subcutaneous injection of ISO can induce myocardial injury in rhesus monkeys. The comprehensive indexes of cardiac ultrasound, electrocardiogram and hematology can be used as the evaluation basis for the model of non-invasive cardiac injury in rhesus monkeys.