动物造模,基因敲除小鼠模型 z-bio 2022-10-20 374

　　Objective To establish a liver specific LCMT1 gene knockout mouse model.

　　Methods The liver specific LCMT1-KO mice were constructed by CRISPR/Cas9 technique. The differences between wild and knockout mice were identified and compared by RT-PCR, real-time quantitative PCR, Western Blot and HE staining; Observe and analyze the general situation, reproductive capacity and offspring survival rate of the two groups of mice.

　　Results The genotype of offspring was successfully identified; The liver LCMT1 mRNA and protein levels of LCMT1-KO mice were significantly lower than those of control mice; There was no significant difference in diet, drinking water, body weight, reproductive capacity, offspring survival rate, liver appearance and HE staining between the two groups; The expression of LCMT1 in the heart, brain and kidney of LCMT1-KO mice had no significant change compared with the control group; The sgRNA of LCMT1-KO mice did not miss the target.

　　Conclusion The successful construction of liver specific LCMT1 gene knockout mice provides an experimental means for studying the regulatory role of LCMT1 gene in diseases.