动物造模,急性单核细胞白血病小鼠模型 z-bio 2022-10-20 381

　　Objective To investigate the effect of silencing the expression of heme oxygenase-1 (HO-1) on leukemic disease in mice with acute monocytic leukemia.

　　Methods U937 cells of different treatment groups were subcutaneously injected into the armpit of NOD/SCID mice to establish the model. The model was divided into blank group and experimental group (U937 group, HO-1 group, empty body group). After the identification model was successfully constructed, the tumor formation time, tumor volume, tumor invasion to surrounding tissues, peripheral blood leukocyte and platelet count, hemoglobin content and survival time of different groups of mice were compared.

　　Results Fluorescence microscope and Western blot confirmed that lentivirus was successfully transfected. Compared with the blank group, in the experimental group, a mass was formed under the armpit, the peripheral white blood cell count was significantly increased, and leukemic cells were seen on the smear, and a group of cells were found in the bone marrow fluid expressing CD13, CD14, and CD64, which confirmed the success of the modeling. Compared with U937 and empty body group, the time required for the M5 mouse model to develop subcutaneous mass in the silent HO-1 group was significantly longer (P<0.05), the growth rate of the mass was slower (P<0.05), the number of white blood cells in the peripheral blood increased and the platelet decreased more slowly (P<0.05), and the survival time was significantly longer (P<0.05).

　　Conclusion Silencing the expression of HO-1 can slow down the progression of leukemia in M5 mouse model, and HO-1 may become one of the therapeutic targets of M5.