动物实验,IGF-1基因 z-bo 2020-08-21 486

　　Objective: To study the effect of IGF-1 gene therapy on the rat model of postpartum stress urinary incontinence, and to explore the ideal treatment method for the disease.

　　Method: 240 SD rats were modeled by the water sac vagina expansion method. 148 SD rats were randomly selected from 185 successful models and divided into 5 groups. IGF-1 gene therapy, amino antagonist therapy, with or without electrical stimulation therapy , Empty plasmid vector injection and no treatment, another 20 unmodeled rats were selected as the blank control group, urodynamic test and serum biochemical index (LDH, on the 1, 21, 42, 63 days after treatment in each CK in the group). Detect and observe the changes of pubococcygeal muscle fibers with an optical microscope.

　　Result: The treatment effect of the IGF-1 group and the electrical stimulation group was improved 21 days after treatment, regardless of the maximum bladder volume, leakage point pressure, and contractile force/muscle weight ratio. There was no significant difference between group 1 and electrical stimulation group (P\→0.05).

　　Conclusion: IGF-1 gene therapy and electrical stimulation therapy are better than other groups, such as drug treatment of rats with postpartum stress urinary incontinence.