动物造模 z-bio 2022-10-31 301

　　Objective To establish oligodendrocyte specific knockout fibroblast growth factor 9 (FGF9) mouse model and further study the role of FGF9 in neural development.

　　Methods Olig1 Cre transgenic mice were hybridized with FGF9 transgenic mice (FGF9flox/flox). Female FGF9flox/wt/Olig1 Cre+was mated with male FGF9flox/flox, and oligodendrocyte specific FGF9 knockout mice (FGF9flox/flox/Olig1 Cre+) were obtained from F3 generation. In order to verify the specificity and effectiveness of conditional gene knockout, genomic DNA of rat tail tissue was extracted, its genotype was identified by PCR technology, and the expression of FGF9 protein was verified by protein electrophoresis and laser confocal, and its phenotype was observed.

　　Results FGF9flox/flox/Olig1 Cre+mice were successfully constructed at gene and protein levels. Preliminary phenotypic analysis showed that the knockout group mice were viable and fertile, with the same survival period as the control group, but with slow development and significant weight loss.

　　Conclusion FGF9 gene knockout mice in oligodendrocytes were successfully obtained. Conditional knockout of FGF9 gene caused slow development of mice.