动物造模,肝双重动脉血供模型 z-bio 2022-11-01 243

　　Objective To establish a stable and successful rat model with 70% hepatectomy and double hepatic artery blood supply under microscope.

　　Methods 76 rats were included in the study, which were divided into training group (40 rats) and model group (36 rats). The training group simulated the operation and optimized the perioperative management; In the model group, 70% hepatectomy (left lobe and middle lobe of liver)+hepatic double artery blood supply operation were prepared under the microscope. After the operation, the general situation of rats was observed, and the success rate of modeling and 1-week survival rate were calculated.

　　Results The training group simulated the operation and crossed the learning curve. The operation time of the model group was (65.3 ± 6.56) min, and the vascular anastomosis time was (11.1 ± 2.53) min. There was no obvious side injury during the operation, and the amount of bleeding was (0.6 ± 0.21) mL. One week later, the success rate of modeling was 88.9% (32/36); The rats were in good condition after operation.

　　Conclusion The operation of preparing 70% hepatectomy+LDABS model of rats under microscope is relatively complicated, but after a certain period of microsurgical skill training, the model has a high success rate and strong repeatability through the improvement and innovation of the surgical methods, especially vascular anastomosis and perioperative nursing, which lays a foundation for further research on its application in liver regeneration, acute liver failure and liver transplantation.