动物造模,肝癌原位移植性肿瘤动物模型 z-bio 2022-11-01 397

　　Objective To establish an efficient and stable animal model of orthotopic liver transplantation tumor.

　　Method 10 μ L Microinjection needle will μ L contains 5 × 105 H22 cell solutions were implanted in the left liver lobe of mice along the long axis of the liver lobe. On the key step of withdrawing the needle to avoid the leakage of H22 cells, cotton pressing and heat sealing methods were used respectively. On the 19th day after inoculation, the size, weight and pathological changes of liver tumors were detected, and the differences between the two methods in the preparation of stable and uniform orthotopic liver transplantation tumors were compared.

　　Results The liver tumor formation rate of the two methods was 100%. Although there was no statistical difference in tumor size, weight and pathological performance between the two groups, compared with the cotton compression group, the size of liver transplantation tumor in the heat sealing group was uniform, and the fluctuation of tumor weight was small. In particular, at the time of autopsy, it was found that high rates of ascites (36.4%) and abdominal aneurysm (36.4%) were found in the cotton compression group, while no such phenomenon was found in the heat sealing group, with significant statistical difference between the two groups (P<0.05). This shows that the leakage of tumor cells from the injection hole in the cotton compression group is significantly increased, which is also the fundamental reason for the large fluctuation of the size of liver transplantation tumor in the cotton compression group. In addition, one mouse with ascites was also found in the cotton compression group to have multiple tumor nodules in the liver, which may be the liver metastasis of ascites tumor cells through the vascular system.

　　Conclusion This study successfully explored a set of technical methods and implementation points for preparing homogenous and stable orthotopic liver transplantation tumor, which has the characteristics of short time, easy operation, stable model, etc., providing a good reference for relevant researchers.