动物造模,胆酸诱导急性肺损伤小鼠模型 z-bio 2022-11-01 287

　　Objective To establish a mouse model of acute lung injury induced by cholic acid, screen the drug delivery mode, and explore the feasibility of lung injury induced by cholic acid.

　　Method According to the administration method × Drugs (3 × 3) Factorial design: mice were divided into groups. The administration methods included tracheotomy, nasal drip for 1 day and nasal drip for 6 days. The drugs were cholic acid and its dissolution control DMSO and blank control PBS. Therefore, there were 9 groups of mice in total. Body weight changes were monitored during the administration period. After the administration, the chest was examined by X-ray plain film, pathological changes of lung tissue were observed histologically, arterial blood was taken to analyze partial pressure of blood oxygen (PO2), and tumor necrosis factor (TNF) in lung tissue was detected by ELISA- α) And interleukin-1 β (IL-1 β) Content of.

　　Results In the tracheotomy and reperfusion group, the X-ray plain film showed diffuse infiltration of lung tissue, obvious hemorrhage in lung gross samples, and histopathology showed a large number of inflammatory cells infiltration and thickening of alveolar wall, decreased partial pressure of blood oxygen, and TNF- α And IL-1 β It was significantly higher than other groups. The changes of each index in the bile acid group after intranasal drip for 1 day and the bile acid group after intranasal drip for 6 days were less than those in the tracheotomy reperfusion bile acid group.

　　Conclusion Tracheotomy and cholic acid infusion can successfully establish acute lung injury model in mice.