动物造模,慢性高尿酸血症肾损害大鼠模型 z-bio 2022-11-01 366

　　Objective To establish a rat model of chronic hyperuricemia induced renal damage, and to provide a model tool for drug development against chronic hyperuricemia induced renal damage.

　　Methods Forty male SD rats were randomly divided into five groups: normal group, group A (fed with 2% potassium oxazinate+12% yeast extract+86% common feed), group B (fed with 0.15% adenine+10% yeast extract+89. 85% common feed), group C (fed with 100 mg/kg adenine+1500 mg/kg potassium oxazinate once a day in the morning and evening) and group D (fed with 50 mg/kg adenine+1500 mg/kg potassium oxazinate once a day in the morning). The observation time was 5 weeks. The body weight, serum uric acid, creatinine, urea nitrogen and other indicators of rats in each group were measured every week. After 5 weeks, the ratio of kidney weight to body weight was measured, and the kidneys were observed by pathological sections.

　　Results Compared with the normal group, the weight of rats in group C decreased, the ratio of kidney weight to body weight increased, the serum uric acid, creatinine and urea nitrogen increased, the color of kidneys changed significantly, and HE and urate staining showed that the kidneys were damaged to some extent.

　　Conclusion 100 mg/kg adenine+1500 mg/kg potassium oxazinate is the best way to establish chronic hyperuricemia renal damage model in rats.