动物实验,2型糖尿病 z-bo 2020-08-21 515

　　Objective: To explore the relationship between two-color alcohol extracts of coreopsis, intestinal flora and type 2 diabetes (T2DM).

　　Methods: Collect model group (A), coreopsis dichromol extract high-dose group 1.8 g/kg (B), coreopsis dichromol extract equal dose group 1.2 g/kg (C), metformin control group 0.2 g . Use / kg (D), blank control group (E), 16SDNA real-time fluorescent quantitative PCR technology, Pearson analysis to detect Clostridium sphaericus and Bacteroides polymorpha in stool samples. We used to determine the level of target bacteria and analyze the correlation of fasting blood glucose (FBG) in each group of mice.

　　Result: (1) The content of Clostridium sphaericus and polymorpha in the model group was higher than that in the normal group (P = 0.017, P = 0.002). (2) Compared with the model group of the large-dose 2-color large intestine-coated alcohol extract group, the content of Bacillus sphaericus and Bacteroides polymorpha decreased, and there was a statistical difference (2 weeks: P = 0.027, P = 0.006, 4 Week: P = 0.007, P = 0.012). (3) The content of Bacillus sphaericus and Bacteroides polymorpha is positively correlated with the content of FBG.

　　Conclusion: The alcohol extract of two-color coreopsis has a certain effect on the intestinal flora of type 2 diabetic mice, and it is related to the blood sugar level.