动物造模,慢性血栓小鼠模型 z-bio 2022-11-01 322

　　Objective To screen the conditions of chronic thrombosis model in mice by continuous intraperitoneal injection of carrageenan solution with different concentrations.

　　Methods 70 male ICR mice were randomly divided into 7 groups, 10 in each group, namely: blank control group, 0.02% dose group, 0.04% dose group, 0.06% dose group, 0.08% dose group, 0.10% dose group, 0.20% dose group. The mice in the blank group were intraperitoneally injected with normal saline (0.01 mL/g), and the other groups were intraperitoneally injected with carrageenan solution of corresponding concentration (0.01 mL/g), once a day; The mice in each group were fed at room temperature of (20 ± 1) ℃ and fed and drank freely. Observe the occurrence of black tail in each group every day (black tail rate, black tail length); Sampling immediately after the bolt is out; Blood samples were collected to detect four items of blood coagulation, and indicators of oxidative stress and inflammatory factors in colon tissue and plasma were detected.

　　Results In the 0.06% dose group, 2 mice were embolized on the 5th day and 8 on the 6th day; Compared with the blank control group, the FIB content in the 0.06% dose group was significantly increased (P<0.05), APTT was significantly shortened (P<0.05), TT was significantly prolonged (P<0.05), and PT was extremely significantly prolonged (P<0.01); In the 0.06% dose group, SOD and GSH Px in colon tissue and plasma decreased significantly (P<0.01), while MDA increased significantly (P<0.05); TNF in colon tissue and plasma of 0.06% dose group- α、 IⅡL-1 β It was significantly increased (P<0.05) and IL-10 was significantly decreased (P<0.05).

　　Conclusion At room temperature of (20 ± 1) ℃, 0.06% carrageenan solution (0.01 mL/g) was injected intraperitoneally for 6 days (once a day).