动物造模,化疗损伤性卵巢早衰模型 z-bio 2022-11-01 339

　　Objective To explore the method of establishing the model of chemotherapy-induced premature ovarian failure in rats by intraperitoneal injection of different doses of cisplatin (CP).

　　Methods Forty eight female infertile Wisar rats were divided into 6 groups with 8 rats in each group. Different doses of CP were injected intraperitoneally at different time points. The control group (Group A) had no treatment; CP was injected intraperitoneally in small dose (1.5 mg/kg) group 1 (group B), small dose (2.0 mg/kg) group 2 (group C) and small dose (2.5 mg/kg) group 3 (group D) once a day for 5 consecutive days; CP was intraperitoneally injected with large dose (group 1, group E), 4 mg/kg, and then injected again at an interval of one week (4 mg/kg); CP was intraperitoneally injected with large dose (group F, group 2), 4 mg/kg, and 6 mg/kg was injected every other week to establish the model of chemotherapy-induced POF in rats. The general condition and estrous cycle of rats were observed, and the body mass change, serum anti Mullerian hormone (AMH) level, ovarian pathological changes and the number of follicles at all levels were detected.

　　Results Compared with group A, the estrous cycle of rats in each treatment group was obviously disturbed, the body mass was significantly decreased, and the serum AMH level was also significantly reduced (P<0.05), especially in group E; The number of follicles at all levels in the ovaries of rats in each model group decreased significantly (P<0.05), especially in group E and group F (P<0.01).

　　Conclusion The model of POF induced by chemotherapy is better in rats by intraperitoneal injection of 4mg/kg CP and re injection every other week, and the method is simple.