动物造模,肥胖模型 z-bio 2022-11-01 470

　　Objective To observe the effect of establishing rat obesity model with high calorie diet, and the difference between the two kinds of rats, and compare different obesity evaluation methods and obesity sensitive animal screening methods, so as to provide reference for preparing animal models when studying the function of weight loss products.

　　Methods 120 SD and Wistar male rats were selected and randomly divided into two groups (blank control group and model group) according to body weight, with 60 rats in each group. The blank control group was given maintenance feed, and the model group was given high calorie feed with improved formula. The body weight and body length of rats were recorded once a week, and the feed quality was weighed twice a week. Lee's index, food intake and food utilization rate were calculated for 6 weeks. After feeding for 2 weeks or 6 weeks, 30 rats in each group were selected to test the blood biochemical indicators. After dissection, bilateral perirenal fat and peritesticular fat were taken and weighed, and the fat/body ratio was calculated. To analyze the correlation between the increase of body weight, Lee's index and obesity and obesity sensitivity, and compare the correlation between the increase of body weight and obesity in rats whose body weight was in the last 1/3, 10%, and 20% respectively after 2 weeks of feeding.

　　Results Compared with the blank control group, the body mass, Lee's index, body mass, food utilization rate, body fat mass and fat/body ratio of the two strains of model group rats increased, the food intake decreased, the levels of serum glucose and triacylglycerol increased, and the level of high-density lipoprotein cholesterol decreased (P<0.05). compared="" with="" wistar="" there="" was="" no="" significant="" difference="" in="" the="" indexes="" of="" model="" group="" rats="" p="">0.05). There were occasional differences in the body length, Lee's index and food utilization rate of the blank control group rats (all P<0.05). The level of serum high-density lipoprotein cholesterol of the blank control group rats was significantly different at 2 and 6 weeks of feeding (P<0.05). The correlation between the increase of body mass and obesity and obesity sensitivity in rats of both breeds was significantly greater than that between Lee's index and obesity and obesity sensitivity (all P<0.05). The correlation between the increase of body mass and obesity was significantly better in rats with 10% and 20% of the increase of body mass excluded than in rats with 1/3 of the increase of body mass excluded, and the correlation coefficient of 10% excluded was the highest.

　　Conclusion The obesity model of SD and Wistar rats can be successfully established with the improved high calorie diet, and there is no significant difference between the two breeds; As an index for obesity evaluation and obesity sensitive animal screening, the increase in body mass is superior to Lee's index, and the method of eliminating the increase in body mass at the bottom 10% can be used for screening obesity sensitive animals.