动物造模,经骨髓注射肺转移和术后复发模型 z-bio 2022-11-02 374

　　Objective to establish the model of lung metastasis and postoperative recurrence by bone marrow injection, and to reveal the superiority and feasibility of bone marrow injection model, so as to provide a new research basis for the study of lung metastasis of malignant tumors.

　　Methods The mice were divided into milk pad injection group, subcutaneous injection group, tail vein injection group and bone marrow injection group. Using 4T1 cells to construct different models, observe the growth, survival and metastasis efficiency of different groups of primary tumors. Different number of 4T1 cells were injected to observe the time of pulmonary metastasis. Postoperative recurrence model was established by injecting 4T1 luc cells into bone marrow. Mice were divided into sham amputation group, amputation group on the third day, amputation group on the seventh day and amputation group on the tenth day. Lung metastasis of mice was observed by living imaging.

　　Results Compared with breast pad injection and subcutaneous injection, bone marrow injection had no effect on the growth of primary tumor, but its survival time was significantly shortened. Compared with breast pad injection, subcutaneous injection and tail vein injection, bone marrow injection has the highest efficiency of lung metastasis. Bone marrow injection only needs 1 × The number of 105 4T1 cells can cause lung metastasis on the 12th day. The model mice injected with bone marrow can still cause lung metastasis and recurrence of primary tumor after amputation.

　　Conclusion The mouse model of lung metastasis by bone marrow injection and the mouse model of postoperative recurrence were successfully established. The mouse model using this injection method has the characteristics of short survival time and high metastatic efficiency, which can be used for the mechanism study of lung metastasis of malignant tumors and drug screening.