动物造模,肝外型胆汁淤积模型 z-bio 2022-11-02 373

　　Objective To evaluate the advantages and disadvantages of three surgical models of extrahepatic cholestasis in rats by serology, imaging and pathology.

　　Methods Fifteen female SD rats were randomly divided into the traditional group, the modified group and the electrotome group. Cholestasis models were established by common bile duct ligation (n=5), common hepatic duct suture (n=5) and high-frequency electrotome coagulation (n=5). Before and after the operation, CT plain scanning and weight measurement were performed to compare the liver density and bile duct diameter; On the 7th day after operation, blood samples were collected from 3 groups of rats, and AST, ALT, TBIL and DBIL were measured; After blood collection, the rats were killed by excessive anesthesia. Some liver tissues were taken and fixed with 4.2%~5.2% formalin solution. The pathological changes were observed by HE staining on paraffin sections.

　　Results On the 7th day after the model establishment, the rats in the three groups had liver injury and cholestasis of different degrees. AST, TBIL and DBIL in the traditional group were higher than those in the electric knife group (P<0.05), and AST, ALT, TBIL and DBIL in the traditional group were higher than those in the improved group (P<0. dbil="" p="">0. 05); Pathology showed that in the traditional group, the degree of bile duct hyperplasia and bile duct wall thickening was the most serious, while in the improved group, the degree was the least, and the electric knife group was between the two groups; The diameter of expanded extrahepatic bile duct in traditional group was larger than that in improved group and electrotome group (P<0. p="">0. 05); The liver CT value in the traditional group was significantly lower than that before operation (P<0. ct="" p="">0. 05).

　　Conclusion Both the electric knife method and the improved method can effectively prolong the course of cholestasis in rats, and can better simulate some human extrahepatic cholestasis diseases, which provides a possibility for dynamic observation of the pathological process of cholestasis.