动物造模,肝纤维化模型 z-bio 2022-11-02 302

　　Objective To compare the effects of different doses of carbon tetrachloride on the induction of liver fibrosis in inbred C57BL/6 mice, so as to establish a stable liver fibrosis model.

　　Methods Five week old C57BL/6 inbred mice were injected intraperitoneally with high, medium and low doses of carbon tetrachloride to induce liver fibrosis. After the experiment, blood and liver tissues were taken to detect the levels of plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver pathological changes.

　　Results After the inbred line C57BL/6 mice were induced by drugs, the animals in each dose group could survive to 8 weeks, and the survival rate in the high-dose group was only 20%, which was far lower than that in the middle and low groups; There was a dose - time effect relationship between the increase of serum transaminase content and pathological changes of liver tissue. Pathological analysis showed that hepatocyte degeneration/necrosis, mixed cell infiltration around central vein/portal area and liver fibrosis were observed in all dose groups.

　　Conclusion 10% carbon tetrachloride can induce inbred C57BL/6 mice to form a stable liver fibrosis mouse model after 8 weeks of administration, which lays a foundation for the follow-up study of liver fibrosis mechanism and drug screening.