动物造模,肺纤维化模型 z-bio 2022-11-02 305

　　Objective To compare the degree of pulmonary fibrosis induced by bleomycin and endotoxin in ICR and C57BL/6 mice, so as to establish a suitable model of pulmonary fibrosis in mice.

　　Methods ICR and C57BL/6 mice were randomly divided into control group, bleomycin group and endotoxin group. Bleomycin group was injected into trachea with bleomycin (5mg/kg body weight), endotoxin group was injected into tail vein with endotoxin (40EU/kg body weight), and control group was injected into trachea with the same amount of normal saline. Eight mice in the control group, bleomycin group and endotoxin group of the two strains were killed respectively on the 7th, 14th and 28th days after the establishment of the model. The general conditions of mice were observed and the lung coefficient of mice was measured; HE staining was used to observe the pathological changes of lung tissue; The content of hydroxyproline in lung tissue was detected by ELISA.

　　Results Compared with the control group, the lung coefficient and the content of hydroxyproline in lung tissue of bleomycin group and endotoxin group of the two strains increased in varying degrees, with significant differences (P<0.05). For the same pulmonary fibrosis inducer, the lung coefficient and the content of hydroxyproline in lung tissue of C57BL/6 strain mice were significantly different from those of ICR strain mice (P<0.05). for="" the="" same="" strain="" of="" there="" was="" no="" significant="" difference="" between="" bleomycin="" group="" and="" endotoxin="" in="" lung="" tissue="" hydroxyproline="" content="" at="" different="" time="" points="" p="">0.05).

　　Conclusion C57BL/6 mice are more sensitive to bleomycin and endotoxin. Both bleomycin and endotoxin were able to prepare the model of pulmonary fibrosis in mice with notional lung injury.