动物造模,帕金森模型 z-bio 2022-11-02 262

　　Objective To establish a stable Parkinson's disease model in C57BL/6 mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and evaluate the behavior of the model.

　　Methods The drug group was established, with the dosage of 35mg/kg, 30mg/kg, 25mg/kg and the control group given 30mg/kg normal saline. The tremor paralysis score, open field experiment, pole climbing experiment, suspension experiment, swimming experiment were conducted respectively, and comprehensive statistics were conducted. The differences between the administration group and the control group as well as between the administration groups were analyzed. At the same time, HE and immunohistochemical TH antibody staining were performed to observe the changes of neurons and nerve fibers in substantia nigra and striatum.

　　Results Compared with the control group, the scores of the administration group were significantly higher, but the behavioral scores of the 25 mg/kg dose group were significantly lower than those of the 35 mg/kg and 30 mg/kg dose groups, and there was no significant difference between the 35 mg/kg and 30 mg/kg dose groups. The number of TH positive neurons in substantia nigra decreased, and the number of TH positive nerve fibers in striatum decreased.

　　Conclusion A simple way to evaluate the effect of Parkinson's disease model in mice by behavioral data has been established.