动物造模,子宫内膜异位症纤维化小鼠模型 z-bio 2022-11-02 236

　　Objective To explore an efficient method to establish and evaluate a mouse model of endometriosis fibrosis.

　　Methods The fibrotic model of endometriosis in BALB/c mice was established by modified intraperitoneal injection; Collect peritoneal lavage solution of mice, and detect TGF in the lavage solution with ELISA kit- β 1 level; The degree of abdominal adhesion was evaluated; HE staining was used to verify whether the endometriosis model was successful; Masson staining was used to detect the deposition of collagen fibers in ectopic lesions and evaluate the degree of collagen deposition; Detection of E-cadherin, Collagen I α- The expression of SMA and smooth muscle myosin heavy chain II (SMMHC-II) to evaluate the degree of ectopic fibrosis.

　　Results The success rate of the improved intraperitoneal injection method in establishing the mouse model of endometriosis fibrosis was 100%. Mouse peritoneal lavage fluid TGF- β 1. The level increased significantly with the prolongation of molding time. Masson staining and immunohistochemical staining of fibrosis related protein showed that there was a fibrosis phenotype in the abdominal cavity of mice with endometriosis.

　　Conclusion The improved intraperitoneal injection method has a high success rate in the construction of mouse endometriosis fibrosis model. The peritoneal lavage solution TGF- β 1 level, Masson staining and fibrosis related proteins E-cadherin, Collagen I α- SMA and SMMHC-II immunohistochemical staining can evaluate the degree of pelvic fibrosis in mice with endometriosis.