动物造模,慢性心肌缺血模型 z-bio 2022-11-02 200

　　Objective To establish the model of chronic myocardial ischemia in mice by subcutaneous injection of different doses of isoproterenol (ISO) into the neck, and to determine the best dose of the model through comparison, so as to seek more objective and reliable indicators, and provide reference for the preparation of relevant models and the evaluation of drug efficacy.

　　Methods Three model groups were injected with ISO 32mg/kg, 16m/kg and 8mg/kg body weight subcutaneously for three consecutive days, and ECG was recorded at 24h, 8d and 15d after modeling; On the 8th and 15th days, the blood myocardial injury markers were detected and the heart samples were taken, and the myocardial morphological and pathological changes were observed.

　　Results The T wave of ECG in the ISO32m/kg and ISO16m/kg dose groups decreased significantly 8 days and 15 days after modeling (P<0.05 or P<0.01); The markers of myocardial injury were significantly changed (P<0.05 or P<0.01). The ISO32 m/kg group had pathological changes, and the mortality rate was 42.5%, which was more suitable for the efficacy evaluation of long-term treatment of anti chronic myocardial ischemia drugs. The mortality rate of ISO16 mg/kg was 25%, which was relatively low and did not cause pathological damage to myocardium.

　　Conclusion The model of chronic myocardial ischemia in mice can be established selectively with ISO 16 or 32 mg/kg according to different experimental expectations.