动物造模,慢传输型便秘模型 z-bio 2023-06-07 703

　　Objective To compare the differences between the models induced by different doses and different administration times, and to explore the experimental parameters for establishing a stable model of Loperamide hydrochloride induced slow transit constipation in mice.

　　Methods Healthy male C57BL/6 mice were used to prepare 0.025, 0.25 and 2.5 mg/mL of loperamide hydrochloride suspension by gavage at 9:00 am and 3:00 pm every day. The effects of different doses and different administration times on the slow transit constipation model of mice were observed through the evaluation of the modeling effect from the detection of intestinal transit rate, fecal volume and other indicators.

　　Results 1) To compare the effects of different doses of loperamide hydrochloride on modeling, in the range of 0.5 mg/kg~50 mg/kg of drug concentration, by comparing the intestinal transit rate between groups, the results showed that compared with the blank group, the 50 mg/kg group significantly reduced the intestinal transit rate and the amount of defecation, with a statistically significant difference (P<0.05); (2) Compared with the control group, the intestinal propulsion rate of mice in the 7th day of modeling was significantly lower than that in the blank group (P<0.05); 10="" 14="" there="" was="" no="" significant="" change="" in="" intestinal="" propulsion="" rate="" at="" and="" days="" p="">0.05).

　　Conclusion In this study, it was found that continuous intragastric administration of 50 mg/kg for 7 days could significantly reduce the intestinal propulsion rate and the amount of defecation in mice, which could create a relatively stable model of colonic transit obstruction constipation in mice, providing valuable data for the exploration of the model of slow transit constipation induced by loperamide hydrochloride.